2 edition of regulation of actin gene expression in Xenopus embryos found in the catalog.
regulation of actin gene expression in Xenopus embryos
Nicholas D. Lakin
Thesis (Ph.D.) - University of Warwick, 1991.
|Statement||Nicholas D. Lakin.|
Direct contributions of Otx2 as a positional tag to global gene regulation in the head organizer in Xenopus embryos Yuuri Yasuoka1, Yutaka Suzuki2, Shuji Takahashi3, Norihiro Sudou 1, Yoshikazu Haramoto 4, Makoto Asashima, Sumio Sugano2, and Masanori Taira1 1Dept of Biol Sci, Grad Sch of Sci; 2Dept of Med Genome Sci, Grad Sch of Front Sci; 3Div of. The Xenopus laevis egg stockpiles mitochondria and mitochondrial DNA along with other organelles and macromolecules during its year-long development ().This active period of mitochondrial biogenesis ceases with the completion of egg development and does not resume until relatively late in embryogenesis, as measured by mtDNA synthesis, cytochrome oxidase activity, and mitochondrial .
Regulatory elements of Xenopus col2a1 drive cartilaginous gene expression in transgenic frogs RYAN KERNEY*,1, BRIAN K. HALL1 and JAMES HANKEN2 1Biology Department, Dalhousie University, Halifax, NS, Canada and 2Museum of Comparative Zoology, Harvard University, Cambridge, MA, USA ABSTRACT This study characterizes regulatory elements of collagen 2 α1 (col2a1) in Xenopus that. For these approaches, fixed embryos must be used, and Xenopus offers many advantages. Xenopus is a vertebrate tetrapod closely related to mammals. The large size of their embryos makes them ideal for imaging patterns of gene expression during development.
Developmental expression of the N-myc downstream regulated gene (Ndrg) family during Xenopus tropicalis embryogenesis CHAO ZHONG 1, YAN-KUAN ZHOU, SHAN-SHAN YANG2, JUN-FANG ZHAO1, XIAO-LONG ZHU1, ZHEN-HUANG CHEN1, PEI-CHAO CHEN2, LI-QUAN HUANG1 and XIAO HUANG*,1 1College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang and 2College of Life . The ashwin gene, originally identified in Xenopus laevis, was found to be expressed first in the neural plate and later in the embryonic brain, eyes, and spinal cord. Functional studies of ashwin suggest that it participates in cell survival and anteroposterior patterning. Furthermore, ashwin is expressed zygotically in this species, which suggests that it participates in embryonic development.
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Analysis of gene expression in Xenopus embryos. Lavery DL(1), Hoppler S. Author information: (1)School of Medical Sciences, University of Aberdeen, Aberdeen, Scotland, UK. Determining the expression pattern of a gene of interest is critical to understanding when, Cited by: 6.
Low levels of transcription from the hisbin clone were Actin Gene Expression in Transformed Xenopus a A?B b I myo act 4f36> > RJJ1J~ MAU I my" ~ end Og AB mrr J0 ,ft 40 Staged embryos Cited by: Tao Q, Lloyd B, Lang S, Houston D, Zorn A, et al.
A novel G protein-coupled receptor, related to GPR4, is required for assembly of the cortical actin skeleton in early Cited by: 6. Xenopus (/ ˈ z ɛ n ə p ə s /) (Gk., ξενος, xenos=strange, πους, pous=foot, commonly known as the clawed frog) is a genus of highly aquatic frogs native to sub-Saharan species are currently described within it.
The two best-known species of this genus are Xenopus laevis and Xenopus tropicalis, which are commonly studied as model organisms for developmental biology Class: Amphibia. Wnt signaling and ciliogenesis are core features of embryonic development in a range of metazoans.
Chibby (Cby), a basal-body associated protein, regulates β-catenin-mediated Wnt signaling in the mouse but not we present an analysis of Cby׳s embryonic expression and morphant phenotypes in Xenopus RNA is supplied maternally, negatively regulated by Snail2 but not Cited by: actin gene expression embryos Department of Biological Sciences, University of Warwick, Coventry, CV4 7AL, UK Received 27 September We have studied the transcription directed by the promoter of a Xenopus borealis cardiac actin gene fused to a globin reporter.
Chibby functions in Xenopus ciliary assembly, embryonic development, and the regulation of gene expression Jianli Shi, Ying Zhao, Domenico Galati1, Mark Winey, Michael W.
Klymkowskyn Molecular, Cellular, & Developmental Biology, University of Colorado, Boulder, COUSA. Developmental control actin of genes in Xenopus (a) appear not to be transcribed during oogenesis or in early embryos.
The con. The FLP-mediated gene activation can be controlled in Xenopus embryos by injecting a mRNA encoding a fusion of FLP to the mutant ligand binding. Expression from DNA Injected into Xenopus Embryos. Authors; Authors and affiliations the method is still under development and few examples of promoter analysis or of ectopic gene expression studies are yet available.
and Gurdon, J. () Upstream sequences required for tissue-specific activation of the cardiac actin gene in Xenopus Cited by: 9. Steinbeisser, H., Hofmann, A., Stutz, F., and Trendelenburg, M.
() Different regulatory elements are required for cell-type and stage-specific expression of the Xenopus laevis skeletal muscle actin gene upon injection X. laevis oocytes and embryos. Nucleic Acids Res. 16, Cited by: 4. Fig. 1 Dissection of early Xenopus tropicalis development by scRNA-seq. (A) Single-cell transcriptomes represent points in a high-dimensional gene expression space.
By collecting single-cell transcriptomes over time of embryo development, it is possible to infer a continuum gene expression manifold connecting cell states across all lineages.
relative order of XmyoD and RSRF gene expresssion in synchronous embryos from blastula to neurula stages, by use of an RNase protection assay (Fig.
Cardiac actin transcription was monitored in the same samples, be- cause in vertebrate embryos this gene is. Dynamic gene expression landscapes of PED. To identify the gene regulation networks that act during in vivo pig PED, we isolated mRNA for deep sequencing from in vivo porcine (Pnm) and mouse embryos (Ms) and porcine SCNT embryos (Pnt) harvested at different stages during PED (Additional file 1: Table S1 and Figure 1A).
At the blastocyst stage, we physically separated the Cited by: same gene now referred to as x-cxcr4b. X-cxcr4b amino acid sequence has a 97% similarity to an x-cxcr4 gene previously cloned and a 60% similarity to z-cxcr4b.
Furthermore, when cxcr4 from mouse, human, rat, zebrafish, and Xenopus were compared, sequence similarity was also very high (Fig. The expression of x-cxcr4b was analyzed by RT-Cited by: 2.
Recent advances in genome editing using programmable nucleases have revolutionized gene targeting in various organisms. Successful gene knock-out has been shown in Xenopus, a widely used model organism, although a system enabling less mosaic knock-out in founder embryos (F0) needs to be explored in order to judge phenotypes in the F0 generation.
mammalian tissues. In the present study, we have examined for the first time the expression of the hsp gene in Xenopus laevis cultured cells and embryos. The Xenopus hsp cDNA encodes an amino acid protein, which shares 74% identity with mice and humans. In Xenopus A6 kidney.
Apoptosis plays an important role in embryonic development. PNAS-4 has been demonstrated to induce apoptosis in several cancer cells.
In this study, we cloned Xenopus laevis PNAS-4 (xPNAS-4), which is homologous to the human PNAS-4 gene. Bioinformatics analysis for PNAS-4 indicated that xPNAS-4 shared % identity with human PNAS-4 and % with mouse PNAS The Cited by: 6.
The expression of ESR-4 and ESR-5 in early Xenopus embryos was determined by whole-mount in situ hybrid-ization (Fig. 2A–D). This analysis indicated that both genes are expressed exclusively in the paraxial meso-derm, in contrast to other Xenopus WRPW–bHLH genes that have sites of expression in both mesodermal and) Xenopus Xenopus embryos.
Anillin is a conserved protein implicated in cytoskeletal dynamics during cellularization and ANLN gene in humans and the scraps gene in Drosophila encode Anillin. Inanillin was first isolated in embryos of Drosophila was identified as an F-actin binding protein.
Six years later, the anillin gene was cloned from cDNA originating from a Drosophila s: ANLN, Scraps, scra, FSGS8, anillin actin. Gene knockdown in Xenopus embryos Embryos were injected at the 4 cell stage with fama -TBMO or fama -SBMO to target the MCCs (B1/B2 lineage), kidney (C3 lineage), LRO (C1/C2 lineage) and Cited by: 2.Evolution and Gene Expression 3 Speciation and post-zygotic isolation 4 Selection on Gene Expression 5 Present methodology for investigating gene expression regulation 8 Mechanisms of Gene Regulation 10 Gene Expression and WGD: Using the Xenopus model 12 Scope of this Thesis 15 References 16 Chapter 2 Title Page Abstract IntroductionAuthor: Dave Anderson.In cases where mammalian gene names reference an original Xenopus name (chordin-like), the Xenopus name will be retained (chordin).
Gene names should not start with any characters or words in order to identify the gene as being Xenopus (e.g. X, Xt, Xl, Xenopus, tropicalis, laevis). Gene names are lower case and italics, and should only contain.